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Ussing chamber study Nonfasted mice were dissected under isoflurane anesthesia, and the cecal tissue was removed and washed well with Krebs Ringer solution (117 mM NaCl, 5.9 mM KCl, 2.5 mM CaCl 2 , 1.2 mM MgCl 2 , 24.8 mM NaHCO 3 , 1.2 mM NaH 2 PO 4 , and 11.1 mM glucose)
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